Empirical assessment of sequencing errors for high throughput pyrosequencing data
نویسندگان
چکیده
منابع مشابه
Quality assessment and control of high-throughput sequencing data
Secondand third-generation sequencing technologies are driving a revolution in biology and medicine, with ultra-high throughput sequencers now able to produce 200 human genomes every 3 days at a cost of $1000 per genome (Watson, 2014). Meanwhile, in our lab at Edinburgh Genomics and in other labs throughout the World, researchers are generating their first single-molecule reads from a hand-held...
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MOTIVATION A ubiquitous and fundamental step in high-throughput sequencing analysis is the alignment (mapping) of the generated reads to a reference sequence. To accomplish this task, numerous software tools have been proposed. Determining the mappers that are most suitable for a specific application is not trivial. RESULTS This survey focuses on classifying mappers through a wide number of c...
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Characterizing the errors generated by common high-throughput sequencing platforms and telling true genetic variation from technical artefacts are two interdependent steps, essential to many analyses such as single nucleotide variant calling, haplotype inference, sequence assembly and evolutionary studies. Both random and systematic errors can show a specific occurrence profile for each of the ...
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Large biological datasets are being produced at a rapid pace and create substantial storage challenges, particularly in the domain of high-throughput sequencing (HTS). Most approaches currently used to store HTS data are either unable to quickly adapt to the requirements of new sequencing or analysis methods (because they do not support schema evolution), or fail to provide state of the art com...
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The DNA in our cells contains our genetic hereditary information, and is literally the blueprint of our body. The functional units of the genome are regions of continuous DNA sequence, and are called genes. According to the Central Dogma of Biology, the DNA sequence of the gene is transcribed into a messenger RNA (mRNA), which is in turn translated to proteins, which perform most tasks in the c...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2013
ISSN: 1756-0500
DOI: 10.1186/1756-0500-6-25